Faithin Omaha eBook Collection

Download or read book New Techniques in Single Molecule Optical Trapping written by James Inman and published by . This book was released on 2014 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical trapping is a powerful single molecule technique used to study dynamic biomolecular events, especially those involving DNA and DNA-binding proteins. The optical trap has the capability to stretch, twist, or unzip single DNA molecules, usually along a single dimension. Access to more dimensions of a single molecule system will be an essential feature in next generation single molecule tools to study more complex bimolecular systems, such as transcription and replication machinery, that stretch, twist, and unwind multiple strands of DNA. To this end, two new techniques for single molecule optical trapping are presented. First, a passive torque wrench is developed to increase the versatility and flexibility of the angular optical trapping technique by passively clamping the toque while simultaneously monitoring the angular orientation of the trapped particle. Second, a novel optical trapping assay is presented that allows simultaneous DNA stretching, twisting, unzipping, and fluorescence of a three-branch DNA construct, the DNA Y structure.