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Download or read book Investigating the Mechanisms of DNA Damage Response in Saccharomyces Cerevisiae written by Jasmine Markita Siler and published by . This book was released on 2018 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The DNA damage repair and checkpoint pathways are a complex web of factors working to ensure genome stability. These pathways become even more complex when considering the protective chromatin barrier that they must navigate. This thesis provides insight into the functions of the Saccharomyces cerevisiae chromatin remodeler Fun30 in DNA damage tolerance (DDT) and DNA damage checkpoint (DDC). It provides new evidence on the cell cycle regulation of Fun30 as well as new information about the levels of Fun30 throughout the cell cycle and how this relates to its function in cellular tolerance to genotoxic agents. The work presented here also places Fun30 in the DDT pathway as an inhibitor of a Rad51-dependent HR pathway, as well as in the DDC pathway as an inhibitor of DDC. This thesis provides new information about known components of both DDT and DDC, thereby improving our understanding of both of these complex processes. Chapter one offers an in-depth introduction to the DNA repair pathways, chromatin remodeling, and the different types of genotoxic reagents used in this work. It also provides background on replication stress, DDT, the DDC, and the replication checkpoint. Chapter two focuses on the functions and regulation of the Fun30 chromatin remodeler. It presents several new findings about Fun30 levels, its cell cycle regulation, and what this means to its function in aiding cellular survival to genotoxic agents. It also has data supporting the idea that Fun30 deletion suppresses the DDT defect observed in cells lacking the Rad5-dependent template switching pathway. This observation is further investigated in this chapter to explore the mechanism involved. Lastly, this chapter presents new evidence for the inhibition of the DDC pathway by Fun30. Chapter three explores the negative regulation of the DDC by Fun30; looking into what other components it may depend on, as well as investigating if it has any similarities to other known inhibitors of DDC. Interestingly, the results in chapter 3 finds new functions in DDC inhibition for previously known DDC activators. Chapter four provides final conclusions that can be drawn from this thesis as well as offering future directions based on outstanding questions."--Pages vii-viii.