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Download or read book Investigating the Genetic Linkage Between Chromosomal Replication and Cell Division in Caulobacter Crescentus written by Duha AlAwad and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Caulobacter crescentus is a well-established model for studying the bacterial cell cycle, a complex process where the stages of growth, chromosome replication, and cell division often overlap, highlighting the presence of a highly coordinated regulatory network that remains partially understood. To search for novel regulators of the cell cycle, specifically of chromosomal replication, we developed a novel molecular screen to isolate dipM-like mutants. DipM is an endopeptidase and a cell division protein that was implicated in the coordination of DNA replication and cell division by our group. We believe that by generating Caulobacter mutants and selecting dipM-like phenotypes, it is possible to target defects in the pathway that regulate the progression from chromosomal replication to cell wall division, where DipM seems to be a key player. Out of nearly a hundred mutants, three dipM-like mutants were selected for genome sequencing, MUT1, MUT2, and MUT3. Bioinformatics analysis of these mutants allowed the identification of six gene candidates that could be linked to the regulation of the bacterial cell cycle, specifically chromosomal replication. We further investigated MUT1 and MUT3 by complementing them with the wildtype (WT) counterparts of the mutated genes to test for WT phenotype restoration. Our findings suggest that the identified gene candidates contribute to the cell cycle progression, specifically to chromosomal replication, by maintaining protein homeostasis. We speculate that DipM works with other regulatory proteins to sense and react to disturbances in protein homeostasis. Overall, our findings provide evidence for the effectiveness of our genetic screening technique and its capacity to detect cell cycle regulators coordinating between chromosome replication and cell wall division"--