Faithin Omaha eBook Collection

Download or read book Dissecting the Targeting Mechanism of the Saccharomyces Retrotransposon Ty5 written by Yunxia Zhu and published by . This book was released on 2001 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interaction was detected between the Ty5 integrase C-terminus and the Sir4p C-terminus. This interaction was greatly reduced by mutations that abolish target specificity. Furthermore, the targeting domain by itself is sufficient for the interaction, although it occurs at reduced levels. The Sir4p interaction domain was narrowed down to amino acids 971 to 1237 through serial deletions. Two amino acid residues, tryptophan 974 and arginine 975, were identified as critical for the interaction. Tethering the SIR4 C-terminus to non-silent chromatin creates Ty5 integration hotspots, suggesting that the interaction between integrase and Sir4p is the driving force behind Ty5 target specificity. These findings may make it possible to do site-specific genome manipulation and to develop better gene therapy vectors, whose integration sites can be controlled.