Identification and Characterization of Novel Alleles Required for Antiviral RNA Interference in Caenorhabditis Elegans
Author | : Stephanie Renee Coffman |
Publisher | : |
Total Pages | : 161 |
Release | : 2015 |
ISBN-10 | : 133946702X |
ISBN-13 | : 9781339467023 |
Rating | : 4/5 (023 Downloads) |
Download or read book Identification and Characterization of Novel Alleles Required for Antiviral RNA Interference in Caenorhabditis Elegans written by Stephanie Renee Coffman and published by . This book was released on 2015 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: Both RIG-I and DRH-1 function in antiviral immunity through the activation of type-I interferon signaling in mammals and antiviral RNAi in C. elegans, respectively. This work investigates the function of DRH-1 in the production of virus-derived siRNAs (vsiRNAs). Deep sequencing of wild-type and drh-1 mutants challenged with Orsay Virus demonstrated that drh-1 mutants produced primary vsiRNAs that are dependent on DCR-1 and the dsRNA-binding protein RDE-4. However, these DRH-1 independent vsiRNAs are predominantly derived from the 5' terminal regions of both OrV RNA1 and RNA2 and were depleted from the internal regions of viral RNA. In contrast, wild-type and other mutant animals did not exhibit a bias for primary vsiRNA biogenesis from the 5' terminal regions. Mammalian RIG-I acts as an ATP-powered translocase, though the biological function of this activity is unknown. Therefore, DRH-1 may function to promote the production of primary vsiRNAs spreading from the 5' terminal regions to the internal and 3' regions of the viral RNA using translocase activity.